Which of the following is NOT a method associated with biotechnology?

Controlled breeding is indeed not classified as a biotechnology method. This approach primarily involves the strategic selection of breeding pairs based on phenotypic traits to enhance certain characteristics in the offspring. While it uses knowledge of genetics and animal husbandry, it does not involve any technological manipulation of the organisms' genetic material and is thus considered a traditional breeding method.

In contrast, the other options are closely linked with biotechnology. Semen sexing involves advanced techniques to separate male and female sperm cells, allowing for more controlled breeding outcomes, particularly in livestock. Embryo transfer allows for the manipulation of embryos to selectively breed animals, enhancing genetic traits across generations. Genetic engineering encompasses various techniques that modify the genetic makeup of organisms at the molecular level, facilitating advancements in agricultural productivity and disease resistance. These methods exemplify the integration of technology and science in manipulating biological systems, which fall under the umbrella of biotechnology.